Title
In Vivo Interactions of Nanosized Titania Anatase and Rutile Particles Following Oral Administration
Authors
Sangeetha Aula,a,b Samyuktha Lakkireddy,a,b Atya Kapley,a,c Neha Hebalkar,d Rakesh Kumar Sharma,e Shantveer G Uppinf and Kaiser Jamil*a
aCentre for Biotechnology and Bioinformatics, Jawaharlal Nehru Institute of Advanced Studies (JNIAS), Hyderabad, Telangana, India
bDepartment of Biotechnology, Jawaharlal Nehru Technological University Anantapur (JNTUA), Anantapuramu, Andhra Pradesh, India
cEnvironmental Genomics Division, Council of Scientific and Industrial Research- National Environmental Engineering Research Institute (CSIR-NEERI), Nagpur, Maharashtra, India
dCentre for Nanomaterials, International Advanced Research Centre for Powder metallurgy and New materials (ARCI), Hyderabad, Telangana, India
eSaveetha Institute of Medical And Technical Sciences (SIMATS), Chennai, Tamilnadu, India and Former Director, Defence Food Research Laboratory (DFRL), Mysore, Karnataka, India.
fDepartment of Pathology, Nizam’s Institute of Medical Sciences (NIMS), Hyderabad, Telangana, India
Corresponding author E-mail address: kaiserjamilgene@gmail.com (K. Jamil)
Article History
Publication details: Received: 09th April 2020, Revised: 10th May 2020, Accepted: 11th May 2020, Published: 15th May 2020
Cite this article
Sangeetha A.; Samyuktha L.; Atya K.; Neha H.; Rakesh K. S.; Shantveer G. U.; Kaiser J. In Vivo Interactions of Nanosized Titania Anatase and Rutile Particles Following Oral Administration. Nano Prog., 2020, 2(3), 11-20.
Abstract
Titanium dioxide nanoparticles (TiO2 NPs) have received growing attention for several biomedical applications such as photodynamic therapy, drug delivery, cell imaging etc. Anatase and rutile are important, naturally occurring polymorphs of TiO2. The present in vivo study in mice was carried out to investigate the effects (hemocompatibility, oxidative stress, genotoxicity and histopathology) of interactions of anatase (5-10 nm) and rutile NPs (600-800 nm) on biological components such as blood, tissues and cells following oral administration. These NPs were found to induce significant reduction in some of the hematological and serum biochemical parameters compared to untreated (p<0.05), indicating potential liver damage. The increase in levels of glutathione (p<0.05) and lipid peroxidation (p<0.05) in liver and spleen of mice treated with anatase and rutile compared to untreated tissues suggest the potential role of TiO2 NPs in inducing oxidative stress. Olive tail moment and %tail DNA were higher in liver and spleen of anatase and rutile treated mice compared with untreated mice (p<0.05) and indicate greater DNA damage in the treated tissues. Histopathological studies in mice treated with anatase and rutile NPs showed (i) liver damage as evident from the ballooning of hepatocytes in liver (anatase); focal portal inflammation, spotty necrosis and interface hepatitis in liver (rutile); (ii) red pulp congestion in spleen; and (iii) mild vascular congestion in lung. In conclusion, distribution pattern and interactions of anatase and rutile NPs with the biological milieu were found to be diverse and the resultant implications were established in distinct ways.
Keywords
Titania nanoparticles; Photodynamic therapy; Hemocompatibility; Oxidative stress; Histopathology; Genotoxicity.
